크로마토그래피 원리의 큰 틀도 마찬가지로 두 상에 대한 분배 차이를 이용하여 분석물을 분리, 정제할 수 있습니다. 다만 크로마토그래피에서 두 개의 상은 하나는 고정하고 다른 하나는 일정 방향으로 이동시켜 사용합니다.
The sample injector is utilized to inject the sample into the HPLC system. To accomplish suitable elution, the sample is Usually dissolved in an acceptable solvent that matches the cell period.
측정 가능한 농도 범위는 컬럼에 의해서도 결정됩니다. 컬럼 충진제의 종류, 입자 지름, 컬럼의 크기에 따라 분리에 최적인 시료 주입량이 크게 다릅니다.
uses an autosampler to inject samples. As opposed to employing a syringe to drive the sample into your sample loop, the syringe attracts sample in the sample loop.
The selection in the column form is determined by the physicochemical Qualities with the analytes remaining separated.
The pump is in charge of delivering the cellular section at a continuing movement amount. This makes sure that the mobile section is continuously fed for the column.
The interface amongst the HPLC plus the mass spectrometer is technically tougher than that inside of a GC–MS as a result of incompatibility of a liquid mobile phase While using the mass spectrometer’s high vacuum need.
Establishing an optimized HPLC strategy includes strategically modifying different parameters to accomplish the very best separation for your precise analytes. click here Key parameters for optimization include things like:
加温することが多かったため「オーブン、ヒーター」と称されるが、現在では周辺気温より低温にするための冷却機能が付いている装置も多い。また、周辺気温付近で使用する場合にも冷却機能は一定の効果がある。
Retention times: Time it's going to take for each analyte to get to the detector, supplying a characteristic fingerprint for identification.
Incorrect cellular section composition: The cell period is answerable for separating analytes. An unsuitable cell period composition can cause analytes to elute much too speedily or bit by bit, resulting in broader peaks.
Because it uses a loop injection, the precision of an HPLC method frequently is much better than a GC technique. HPLC just isn't restricted to volatile analytes, meaning we can examine here a broader range of compounds. Capillary GC columns, Conversely, have additional theoretical plates, and can independent much more complicated mixtures.
The sample injector introduces the sample in the HPLC system. Exact and exact sample injection is critical for getting trusted benefits.
A quantitative HPLC Assessment is frequently much easier than the usual quantitative GC Examination since a hard and fast volume sample loop gives a far more specific and exact injection.